• This second edition includes a collection of antigen production and delivery strategies for vaccine development in veterinary species. New and updated chapters guide readers through protocols for antigen production, experimental antigen delivery and the analysis of immune responses upon vaccination. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

    Authoritative and cutting-edge, Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols, Second Edition aims to be a useful and practical guide to researches to help further their study in this field.

    • Front Matter

    Pages i-xi

    1. An Overview of Veterinary Viral Diseases and Vaccine Technologies
      • Alejandro Brun

    Pages 1-26

    1. Production and Purification of Candidate Subunit Vaccines by IC-Tagging Protein Encapsulation
      • Natalia Barreiro-Piñeiro, Tomás Pose-Boirazian, Rebeca Menaya-Vargas, José M. Martínez-Costas

    Pages 27-40

    1. Elastin-like Polymers as Nanovaccines: Protein Engineering of Self-Assembled, Epitope-Exposing Nanoparticles
      • Alessandra Girotti, Juan González-Valdivieso, Irene Alonso-Sampedro, Sara Escalera-Anzola, Sandra Ramos-Díez, F. Javier Arias

    Pages 41-72

    1. Display of Heterologous Proteins in Bacillus Subtilis Biofilms for Enteric Immunization
      • Claudio Aguilar, Ramona Wissmann, Cornel Fraefel, Catherine Eichwald

    Pages 73-95

    1. Production of Influenza H5 Vaccine Oligomers in Plants
      • Hoang Trong Phan, Udo Conrad

    Pages 97-107

    1. DNA Vaccines in Pigs: From Immunization to Antigen Identification
      • Francesc Accensi, Laia Bosch-Camós, Paula L. Monteagudo, Fernando Rodríguez

    Pages 109-124

    1. Use of Foot-and-Mouth Disease Virus Non-coding Synthetic RNAs as Vaccine Adjuvants
      • Miguel Rodríguez-Pulido, Miryam Polo, Belén Borrego, Margarita Sáiz

    Pages 125-135

    1. Evaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccination
      • Zheyi Li, Sreeja Roy, Charani Ranasinghe

    Pages 137-153

    1. Generation of Replication Deficient Human Adenovirus 5 (Ad5) Vectored FMD Vaccines
      • Gisselle N. Medina, Teresa de los Santos, Fayna Díaz-San Segundo

    Pages 155-175

    1. Recombinant Modified Vaccinia Virus Ankara Development to Express VP2, NS1, and VP7 Proteins of Bluetongue Virus
      • Alejandro Marín-López, Sergio Utrilla-Trigo, Luis Jiménez-Cabello, Javier Ortego

    Pages 177-193

    1. Cloning Strategies for the Generation of Recombinant Capripoxvirus Through the Use of Screening and Selection Markers
      • Hani Boshra, Mahder Teffera, Jinxing Cao, Shawn Babiuk

    Pages 195-207

    1. Using RVFV as a Vector Platform for the Expression of Ruminant Disease Antigens
      • Sandra Moreno, Belén Borrego, Alejandro Brun

    Pages 209-225

    1. Generation and Characterization of Single-Cycle Infectious Canine Influenza A Virus (sciCIV) and Its Use as Vaccine Platform
      • Aitor Nogales, Kevin Chiem, Michael Breen, Marta L. DeDiego, Colin R. Parrish, Luis Martínez-Sobrido

    Pages 227-255

    1. Reverse Genetics for Influenza A and B Viruses Driven by Swine Polymerase I Promoter
      • Brittany Seibert, Stivalis Cardenas-Garcia, Daniela Rajao, Daniel R. Perez

    Pages 257-281

    1. Analysis of the Cellular Immune Responses to Vaccines
      • Nicholas Svitek, Evans L. N. Taracha, Rosemary Saya, Elias Awino, Vish Nene, Lucilla Steinaa

    Pages 283-301

    1. Back Matter

    Pages 303-306

  • Alejandro Brun

    Centro de Investigación en Sanidad Animal (CISA), INIA-CSIC, Valdeolmos, Spain

Veterinary Clinics of North America
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